Final Applied Lab Project-BIOL 103
The Effect of low pH on Enzyme Activity
Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. Recall: enzymes are proteins! To complete this project, it may be useful for you to first review the Scientific Method Tutorial, the OLI module about pH and enzymes, Lab 1 (Introduction to Science) and Lab 4 (Enzymes). As you review Lab 4, you will be reminded that there are several factors that impact enzymatic activity: pH, temperature, and amount of reagent. It is OK to use the same enzyme/substrate/method as you did in lab 4 (but modify the treatment), but you are encouraged to search on-line to find a different enzyme/substrate/method for measuring enzyme activity for your project (include all references).
As you design your experiment for this project, please remember that you are trying to examine how an acidic fluid will modify the outcome of an enzymatic reaction. To successfully complete this project, you will need to identify the question(s) being asked in your experiment and the hypothesis that you are testing. In your experimental design, you must clearly explain what you are doing. That means that you will need to identify the enzyme, the substrate, the acidic fluid used as treatment, the control treatment and the method of measuring enzyme activity, as well as explain your experimental protocol. You must also thoroughly explain how the acidic fluid impacted enzyme activity based on the results from your own experiment as well as knowledge of enzymes and pH from the OLI modules, lab manual and potentially additional information sources.


***Assignment information continued in the attachment***
***My outline is also included in the attachment with what controls, samples and treatments I would like to use***

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Instructions

Final Applied Lab Project
Addresses course outcomes 1-4:
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recognize and explain how the scientific method is used to solve problems

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make observations and discriminate between scientific and pseudoscientific explanations

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weigh evidence and make decisions based on strengths and limitations of scientific
knowledge and the scientific method

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use knowledge of biological principles, the scientific method, and appropriate
technologies to ask relevant questions, develop hypotheses, design and conduct
experiments, interpret results, and draw conclusions

This is the culminating lab assessment in BIOL 103. It is designed to assess your ability to apply
the principles of the scientific method.
The Effect of low pH on Enzyme Activity
Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity.
Recall: enzymes are proteins! To complete this project, it may be useful for you to first review
the Scientific Method Tutorial, the OLI module about pH and enzymes, Lab 1 (Introduction to
Science) and Lab 4 (Enzymes). As you review Lab 4, you will be reminded that there are
several factors that impact enzymatic activity: pH, temperature, and amount of reagent. It is OK
to use the same enzyme/substrate/method as you did in lab 4 (but modify the treatment), but you
are encouraged to search on-line to find a different enzyme/substrate/method for measuring
enzyme activity for your project (include all references).
As you design your experiment for this project, please remember that you are trying to examine
how an acidic fluid will modify the outcome of an enzymatic reaction. To successfully complete
this project, you will need to identify the question(s) being asked in your experiment and the
hypothesis that you are testing. In your experimental design, you must clearly explain what you
are doing. That means that you will need to identify the enzyme, the substrate, the acidic fluid
used as treatment, the control treatment and the method of measuring enzyme activity, as well as
explain your experimental protocol. You must also thoroughly explain how the acidic fluid
impacted enzyme activity based on the results from your own experiment as well as knowledge
of enzymes and pH from the OLI modules, lab manual and potentially additional information
sources.
Hint: Keep in mind that the acid will change the environmental conditions of the experiment (for
example, a low pH value could change the shape of the active site on the enzyme protein),
without directly participating in the reaction.

Lab Materials
You may need all or some of the following, depending on your experimental design:
Materials from your lab kit:
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pH paper

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hydrogen peroxide solution (you can purchase this at a pharmacy if you have used up the
bottle that came with the lab kit)

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plastic beakers or cups

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vinegar

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yeast (can be purchased at grocery store if more is needed)

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balloons

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plastic bottle

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marker for labeling of beakers

You may choose to use additional materials (different acidic fluids and/or different organisms
and/or different substrate if you chose an enzyme other than catalase).
Outline (10 points) submit in assignment folder in week 7.
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Name of enzyme you will use: Yeast

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Name of organism (if applicable): N/A

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The substrate: Hydrogen peroxide

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Method for measuring enzyme activity: Circumference of balloons

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Treatment: acidic fluid(s), pH, length of exposure, how you will treat your samples:
length of exposure will be 1min, 3min, 5min

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The control(s) in the experiment: 3 Tubes filled with 10ml of catalase, 10ml of hydrogen
peroxide and no acetic acid

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Sample size: 3 tubes with 10ml of catalase, 10ml of hydrogen peroxide and each one
with 1ml, 3ml, 5ml of acetic acid

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Hypothesis: When acetic fluids are added to enzyme-substrate mixtures, enzyme activity
is affected.

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How you will present your data (table and/or type of graph): Table

Write a lab report that includes the following:
1. Title page: descriptive title, your name, course name, semester
2. Introduction: general background information about enzymes and specific information about
your chosen enzyme, the question(s) that you are asking and a clear hypothesis for your
experiment (15 points).
3. Design an experiment. Provide a detailed description of the materials and methods used to
conduct the experiment. Identify control and experimental samples, as well as independent and

dependent variables. Also include the methods used for data collection and analysis (20 points).
4. Conduct the experiment and record your results. What did you observe? Present your data in
table and/or graph format. Remember to label everything and include the unit of measure with
all numbers (20 points)
5. Use your knowledge of enzymes and pH to interpret and discuss your results. It may be
necessary for you to refer to the OLI course modules, lab manual and/or use additional
information resources. What effect does the acidic treatment have on enzyme activity? Did you
get the expected results? Explain. (15 points)
6. State a specific and accurate conclusion. Is your hypothesis supported by the results? Looking
back, how could you have improved your experiment? (10 points)
7. Include a list of references to all information sources used in APA format (5 points).
Avoid Plagiarism
It is very important to write with your own words. If you do copy one or two sentences directly
(use sparingly), use quotation marks (") around the copied text, followed by an in-text reference.
All information sources need to be included in the reference list and as in-text references.
Plagiarism will be reported to the proper UMUC authority. Guidance on how to avoid plagiarism
can be found here: UMUC's How to Avoid Plagiarism
Submission
Submit your final applied lab project to both the TurnItIn website as well as in the assignment
folder by the due date specified in the course schedule.
Grading
Your Final Applied Lab Project will be graded based on the following criteria (100 points total):

Hide Rubrics

Rubric Name: Final Applied Laboratory Project
Criteria

Excellent (90100%)
100 points

Good (75-89%)

Outline 10 points Outline includes all Outline missing one
(Due in week 7) the required
or two of the
components and
required
planned experiment components,
meets requirements planned experiment
for the assignment does not meet one of
and is clearly and
requirements for the
accurately described. assignment
Submitted on time. minor issues with

9-10 points

Introduction,
Question and
Hypothesis (15
points)

clarity and accuracy.
7-8 points

Background
Background
information about
information about
enzymes in general
enzymes in general
and about specific
and about specific
enzyme is somewha
enzyme used in
unclear and/or
project is clearly and
inaccurate.
accurately written.
Questions and
Questions and
hypothesis could be
hypothesis are
more specific,
specific, relevant and
relevant and clearly
clearly stated.
stated.
14-15 points
11-13 points

Experiment is mostl
designed to test the
Experiment is
hypothesis
designed to directly description of
test the hypothesis; experiment is
description of
somewhat inaccurate
Experimental
experiment is
and/or
Design, Materials detailed and well
information about
and Methods (20 written and includes materials and
points)
all materials and
methods used is
methods used.
missing
minor problems with
clarity an
18-20 points
organization.
15-17 points

Results are clearly
and accurately
Results (20 points) presented in a table
and/or graph format.
18-20 points

Results are
presented, but minor
problems with clarit
and/or accuracy.
15-17 points

Discussion of
A clear, accurate and Minor problems with
Results (15 points) well organized
clarity, accuracy or

discussion of results
that demonstrates
good knowledge of
enzymes.
14-15 points

Conclusion ( 10
points )

organization
demonstrates some
gaps in knowledge
of enzymes.
15-17 points

A well stated
conclusion that is
accurate and specific
and directly related
to the data collected.
Clearly stated
whether or not the
results support the
hypothesis. Relevant
suggestion for
improvement of
experiment.

Conclusion stated,
but slightly
inaccurate,
not directly related
to data collected
and/or
for improvement of
experiment not very
relevant.
7-8 points

9-10 points
All references are
All references used
included, but a few
are included and in
minor mistakes in
correct APA format,
Citations (5 points) in-text references are APA format,
missing in-text
included.
references.
5 points
4 points
Less than 3 minor
Grammar and spelling and
Spelling (5 points) grammatical errors
5 points
Overall Score

Level 4
90 or more

Between 4 and 7
minor spelling and
grammatical errors
4 points
75 or more

Additional Requirements 

Min Pages: 5 
Max Pages: 7 
Other Requirements: All instructions are in the attachment. Considering it is a lab report, it will probably need to be around 7 pages; 1 for the title, 1 for the references, 5 for the introduction, objective, materials required, hypothesis, hypothesis testing, method, step-by-step methodology, results, discussion (data analysis, observation) and conclusion. This assignment, of course, needs to be original...no plagiarism.