AP-2 Lab 09 – Lab Report Name:…
AP-2 Lab 09 – Lab Report Name:... AP-2 Lab 09 – Lab Report Name: ____________________ Section: ___________________ Lab Report: Exercise 9: The Digestive System Purpose: Please explain the purpose of this lab. Include in your explanation the major concepts you learned and any safety concerns associated with the lab. Activity 1: Microscopic Observation of Digestive Structures Observations: Be sure to include written descriptions of the tissues and structures you observed in addition to the sketches Simple Columnar Epithelium of the Stomach: Simple Columnar Epithelium of the Duodenum: Ileum Slide: Salivary Gland Slide Liver Slide: Pancreas Slide: Wall Layer Major Functions Mucosa Submucosa Muscularis Externa Serosa or Advantitia Questions: Compare the columnar epithelium from the stomach to that of the duodenum. How is each modified to carry out a specific function? What are the structural modifications you saw in the salivary glands that allow them to carry out their function? Where can you find Kupffer cells and what do they do? How is the ileum structurally different from the duodenum? What is the alimentary canal? Activity 4: Digestive Enzymes and Physiology Observations: Data Table 1: Carbohydrate Digestion Tube Observations (Glucose Test) 1: Amylase, Cracker, 37oC 2. Amylase, Cracker, 22oC 3. Distilled Water, Cracker, 37 oC 4. Distilled Water, Cracker, 22oC Data Table 2: Protein Digestion Tube Observations 1. Trypsin, BAPNA, 37oC 2. Trypsin, Distilled Water, 37oC 3. Trypsin, BAPNA, 22oC 4. Trypsin, Distilled Water, 22oC Data Table 3 A: Controls and Emulsification Tube Observations 1. Oil and Water 2. Oil, Water and Bile Salts 3. Pancreatin and Distilled Water 4. Congo Red Cream and Distilled Water Data Table 3B: Pancreatin Digestion Tube Observations (Pink/Blue) Digestion Occur? 1.Pancreatin, Congo Red Cream, 37oC 2. Pancreatin. Congo Cream, 22oC 3. Pancreatin, Congo Cream, Bile Salts, 37oC 4. Pancreatin, Congo Cream, Bile Salts, 22oC Questions: What is hydrolysis? Complete the following table: Enzyme Organ Producing it Site of Action What does it work on? (Substrate) Optimal pH Salivary Amylase Trypsin Pancreatic Lipase How does BAPNA work? You ran both a body temperature and room temperature test for each enzyme you tested. Did the two temperatures seem to have an effect on the enzyme activity? Why or why not? Why did you have control tubes in each experiment? Explain how salivary amylase works on foods like crackers? Explain how trypsin works on proteins? Fat digestion requires two steps? What are the steps and what enzymes are used to accomplish each ?