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```9.	Suppose your sodium meter reading is 80 mV. Using the standard curve (see last page), calculate your sodium clearance using the flow rate from question 2.
I got a flow rate of 6.45 mL/min for question 2.
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Name: ______________________________

NPB 101L, Quiz 6

Instructions:
You may use any written references you deem necessary (e.g. lab manual, textbooks)
but you MAY NOT consult with other students (past or present), other TAs, professors,
neighbors, relatives, pets, extraterrestrials, friends (real or imaginary), beings from
another plane of existence, etc.
1. Your weight is _______ lbs, which is equal to _______ kg. The lab manual (pg 67)
specifies how much you should drink based on your weight. Calculate the four
volumes. You only need to show details of the calculations for one of the
experimental solutions.
a. Volume of distilled water you should drink:

_____________

b. Volume of isotonic saline you should drink:

_____________

c. Volume of bicarbonate you should drink:

_____________

d. Volume of fluid you should drink if you are the control:

_____________

2. The last time you urinated was at 10:40 AM. It is now 1:15 PM. You completely void

3. Assume your flow rate is 2.75 mL/min. Estimate the creatinine concentration of your
urine. Use the estimates of GFR and plasma creatinine concentration given in the

4. In order to accurately measure creatinine concentration using a spectrophotometer,
you must dilute your estimated creatinine concentration to 30 mg/L. Using the value
of estimated creatinine concentration from question 3, calculate the required dilution
factor in order to wind up with a urine creatinine concentration of 30 mg/L. Round

1

Name: ______________________________

NPB 101L, Quiz 6

5. Assuming you start with 1 mL sample of urine, how much distilled water should you
add in order to obtain a dilution factor of 12?

6. Suppose your optical density reading from the spectrophotometer is 1.1. Assume the
standard curve to estimate the creatinine concentration of your DILUTED urine
sample results in the following equation:
y = 0.1411x – 0.0236
where:

x is the creatinine concentration

a. Calculate the creatinine concentration of your DILUTED urine sample.

b. Using the dilution factor calculated in question 4, find the creatinine

7. Using the undiluted urine creatinine concentration you calculated from question 6
and the flow rate from question 2, calculate your creatinine clearance.

8. Is the creatinine clearance rate you calculated in question 7 closed to the expected
normal value for a healthy adult? If not, what should it be approximately (include
units)?
9. Suppose your sodium meter reading is 80 mV. Using the standard curve (see last
page), calculate your sodium clearance using the flow rate from question 2.
10. At what times should you prepare your diluted urine samples for creatinine analysis?
2

Name: ______________________________

NPB 101L, Quiz 6
y = 25.927Ln(x) - 56.3

Na+ Meter #1
150

Voltage, (mV)

100

50

0

-50

-100
1

10

100

1000

Concentration, (mEq/L)

SPEC. A

y = 0.1411x - 0.0236

1.6
1.4
1.2

1
0.8
0.6
0.4
0.2
0
0

2

4

6

-0.2
Concentration, (m g/dL)

3

8

10

12```