bio156 quiz 3
Subject: Biology   / General Biology
Question
1 of 25
What property of a compound light microscope enables a person to change objectives without major focusing adjustments?
resolving power
magnification
parfocal
focusing
resolution
Question
2 of 25
How does closing the iris diaphragm affect image brightness and contrast?
decreases both
increases both
decreases brightness and increases contrast
increases brightness and decreases contrast
decreases brightness but has no effect on contrast
Question
3 of 25
Which of the following objectives has the shortest working distance?
4X
10X
45X
100X
Question
4 of 25
What effect will changing from the low power objective to the high power objective have on the field of view of a specimen?
increase
decrease
no effect
Question
5 of 25
If the letter p is placed under the microscope in the normal reading position, which of the following orientations of the letter would the viewer see?
b
p
q
d
Question
6 of 25
Which of the following procedures should be done just prior to adjusting the diopter, when using the compound microscope?
Clean all lenses with lens paper
Turn the light on
Adjust the iris diaphragm
Adjust the fine focus
Question
7 of 25
Two parts of the microscope that regulate the amount of light visible through the ocular are the:
fine and coarse adjustment knobs
ocular and objective lenses
illuminator and revolving nosepiece
condenser and iris diaphragm
Question
8 of 25
Initial focusing of any slide is done under the:
scanning or low power objective
high power objective
oil immersion objective
depends upon the size of the specimen
Question
9 of 25
Under which magnification would the distance between the objective and the specimen being observed be the least?
40X
100X
450X
1000X
Question
10 of 25
What is the total magnification produced by a microscope, using a 10X ocular lens and a 10X objective lens?
10X
20X
100X
1000X
Question
11 of 25
Which of the following describes proper microscope care and technique.
Be sure to carry the microscope upright, with one hand on the arm and the other under the base.
To protect the optics of the microscope, place it down gently and don’t drag it across the tabletop.
Always begin focusing with the lowest power lens available.
Only use the coarse focus knob with the scanning and low power lenses.
All the above are examples of correct microscope care and technique.
Question
12 of 25
Turning which of the following knobs moves the objective lenses the greatest vertical distance?
coarse focus knob
fine focus knob
Question
13 of 25
Resolution is the ability to see two objects as separate.
True
False
Question
14 of 25
Which of the following regulates the amount of light passing through the slide specimen on the microscope stage?
nosepiece
objective lens
iris diaphragm lever
fine focus knob
Question
15 of 25
An increase in magnification causes
a decrease in the field of view.
a decrease in the working distance.
a decrease in the depth of field.
all of the above.
Question
16 of 25
Working distance is the
distance from your house to your job.
distance the microscope nosepiece travels using the coarse focus knob.
distance from the bottom of the objective lens to the specimen.
distance the specimen can travel across the microscope stage.
Question
17 of 25
When adjusting a binocular microscope for differences between right and left eyes, focus the microscope with the fine adjustment while viewing only with the right eye (fixed ocular).
True
False
Question
18 of 25
When using the oil immersion lens, you should raise the objective lenses for easy application of an oil droplet to the slide.
True
False
Question
19 of 25
Why must a small object seen through the ocular lens be centered in the field of view, before changing to a higher power objective lens?
to prevent damage to the objective lens
to compensate for the decrease in resolving power of the lens
to prevent its disappearance due to the decrease in the field of view
to prevent eyestrain
Question
20 of 25
A parfocal microscope allows you to quickly focus using the low power or scanning lens and then, to switch to a higher power lens with only a minimal adjustment of the fine focus knob.
True
False
Question
21 of 25
Why is it necessary to adjust the amount of light after changing objective lenses?
Higher magnification lenses require more light.
Proper lighting is needed to see specimen details.
The lens aperture decreases with higher magnification lenses.
All of the above.
Question
22 of 25
Which objective would you use to locate a specimen on a slide?
lowest power available
high power
oil immersion
any of the ojectives could be used with equal success
Question
23 of 25
Which of the following parts of the microscope would increase the depth of field?
ocular
high power objective
fine focus
low power objective
Question
24 of 25
Depth of field is best demonstrated with
a slide showing the letter e.
a slide containing overlapping threads.
the light turned completely off.
the oil immersion lens.
Question
25 of 25
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When the letter e slide is viewed with the microscope, it appears
right side up.
right side up and backwards.
upside down.
upside down and backwards.
