This is a lab project relating to Enzymes. It is a…

This is a lab project relating to Enzymes. It is a…

This is a lab project relating to Enzymes. It is a...

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UMUC Biology 102/103
Lab 4: Enzymes
INSTRUCTIONS:
•
On your own and without assistance, complete this Lab 4 Answer Form

electronically and submit it via the Assignments Folder by the date listed on your
Course Schedule (under Syllabus).
•
To conduct your laboratory exercises, use the Laboratory Manual that is
available in the WebTycho classroom (Reserved Reading or provided by your instructor)
or at the eScience Labs Student Portal. Laboratory exercises on your CD may not be
updated.
•
Save your Lab4AnswerForm in the following format: LastName_Lab4 (e.g.,
Smith_Lab4). .
•
You should submit your document in a Word (.doc or .docx) or Rich Text Format
(.rtf) for best compatibility.

Experiment 1: Effect of Enzyme Concentration

Table 1: Effect of Enzyme Concentration on the Production of Gas
Tube

Amount
of yeast

1

Balloon
circumference
(cm) After 5
minutes

Final
Circumference
(cm)

Time
Required to
Complete

0.005 g

3

Balloon
circumference
(cm) After 3
minutes

0.05 g

2

Balloon
circumference
(cm) After 1
minute

0.0005 g

Questions
1. What is the enzyme in this experiment? What is the substrate?

2. Did you notice a difference in the rate of reaction in the tubes with different concentrations of
enzymes? Why or why not?

3. What was the effect of using less enzyme on your experiment?

4. Do you expect more enzyme activity if the substrate concentration is increased or
decreased? Draw a graph to illustrate this relationship.

5. Hydrogen peroxide is toxic to cells, yet is a common byproduct of the reactions that occur
inside the body. How cant his compound be changed to become non-toxic (Hint: Look at the
chemical formula of hydrogen peroxide)?

Experiment 2: Effect of Temperature on Enzyme Activity
Table 2: Effect of Temperature on the Production of Gas
Initial Temp. Final Temp.
(°C)
(°C)

Tube

Circumference Circumference Circumference
Final
Time
(cm) After 1
(cm) After 3
(cm) After 5 Circumferenc Required to
minute
minutes
minutes
e (cm)
Complete

Refrigerator
Room
Temperature
Hot Water (~85
°C)
Boiling Water
(~100 °C)

Questions
1. What is the enzyme in this experiment? What is the substrate?

2. How does temperature affect enzyme function?

3. Do plants and animals have an enzyme that breaks down hydrogen peroxide? How could
you test this?

4. How did the boiling water affect the overall reaction?

5. How can enzyme activity be increased?

6. Design an experiment to determine the optimal temperature for enzyme function, complete
with controls. Where would you find the enzymes for this experiment? What substrate would
you use?

7. Draw a graph using balloon diameter vs. temperature. What is the correlation?