Predict salt concentration the protein would come off
Predict salt concentration the protein would come off
Biology
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Suppose you are purifying a protein on a weak cation exchanger (pH 7.5) and it comes off at 300mM salt. predict what salt concentration (lower, same, higher) the protein would come off if you used a strong cation exchanger. explain the logic behind your prediction in terms of the differences between a weak and strong exchange