Number of lipid molecules we added to each cell to make the tethered membrane.

Number of lipid molecules we added to each cell to make the tethered membrane.

There are three questions that I would like to ask, all related to one another:

1) Calculate the number of lipid molecules we added to each cell to make the tethered membrane.
Molarity of lipid = 3mM
Volume added = 8?L

2) Calculate the number of molecules of lipid in the tethered monolayer film on gold.
Area per tethered molecule = 1nm2
Area of gold electrode = 2mm2

3)What is the fraction of added phospholipid that is incorporated into the membrane?