Looking for examples of a created lab.
Looking for examples of a created lab. Design, conduct, and report on an experiment in which you will test the effect of an acidic fluid on enzymatic activity. (Recall: Enzymes are proteins.) If necessary, it may be useful for you to review the Scientific Method lab. Lab Materials You may need the following, depending on your experimental design. Materials in your lab kit: • excess materials remaining from lab 4 (enzymes) Additional materials you may need: • plastic beakers or cups • hydrogen peroxide solution (from Lab 4) • yeast (from Lab 4) • sample of fresh meat, about 1 cm cubed in size (unprocessed and uncooked, e.g., liver, steak, fish, or poultry—particularly organs) • sample of fresh vegetable, about 1 cm cubed in size (e.g., potatoes or other root vegetables) • a pen for labeling the beakers or cups • a ruler with centimeter markings Instructions: 1. Develop a testable hypothesis regarding the effect of an acidic fluid on enzyme activity. 2. Design an experiment to test your hypothesis. Make a list of all the materials you will need to conduct your experiment and then procure them. Record in advance all the steps you plan to take when executing the experiment. Design and prepare data tables in which you will record your data and observations while executing the exeriment. Decide and make note of how you plan to analyze the data you collect. 3. Conduct the experiment. Record any modifications you had to make to the steps in the experiment as you execute them, including details pertaining to amounts or types of materials used. Record all data you collect and observations you make while executing the experiment. 4. Analyze your results (data and observations). As appropriate, calculate means, prepare summary data tables, refine how you will present your data in your report, prepare graphs of data, etc. 5. Use your knowledge of enzymes to interpret your results. It may be helpful for you to refer to your lab manual, textbook, or other sources. 6. Decide whether or not the data you collected supports your hypothesis. Determine whether or not you will accept or reject your hypothesis. 7. Formulate a conclusion about the effect of an acidic fluid on enzyme activity. 8. Prepare and submit a formal written report of your experiment. This will determine your grade for the final applied lab project. See the "report guidelines" below. A detailed grading rubic will be available in the classroom. Report Guidelines: The report must be typed, double-spaced, in Times Roman 12 point font, in paragraph form (not in bulleted or numbered form). It must contain the following sections, clearly labeled, addressing the content identifed below: Introduction: Include background information on the topic of enzyme activity and how it might be affected by an acidic fluid. Cite sources as appropriate. Identify the question you were attempting to answer through the experiment and clearly state your hypothesis. Methods: Provide a detailed account of the materials and methods used to conduct the experiment in paragraph form, not in the form of a numbered or bulleted list). This account should provide sufficient detail for someone else to repeat your experiment. Also include the methods for data collection and analysis. Results: Provide a written summary (in paragraph form) of your results. Include in this section any data tables you have prepared. You must reference in this section all data tables that you include. Do not interpret your results in this section, just present/summarize them. Discussion: Use your knowledge of enzymes to analyze and interpret your results and describe (in paragraph form) that interpretation and analysis in this section. It may be helpful for you to refer to your lab manual, textbook, or other sources. Cite sources as appropriate. Indicate whether or not your hypothesis was supported by the results and whether or not you are accepting your hypothesis or rejecting it. Specifically tie the data you collected to the accepting or rejection of the hypothesis. Conclusion: Briefly state the conclusion about the effect of an acidic fluid on ezyme activity that you arrived at in response to the results of the experiment you conducted.