Lab Exam II BIO230 (Tamrakar)
For Lab Exam II
BIO230 (Tamrakar)
1. Isolation: Various methods of isolation, their features, pros and cons, and the
appropriate use of each method. Know how to perform serial dilutions and
calculate bacterial concentration in a given sample. Know the related calculations
thoroughly. Be able to use the calculations and/or techniques of isolation in an
experiment. Understand the technique of steak plate isolation, and how to perform
it.
2. Capsule staining, Acid-fast staining, endospore staining: Understand the
principle of each type of differential staining. Know the procedures and trouble
shootings. If asked, you should be able to perform it successfully from a given a
culture. Be able to recognize a particular staining if a pre-stained slide is
provided.
3. Control of bacterial growth: Know the mode of action for each of the chemicals
tested in the lab and how they can be used on microorganisms of specific
characteristics. Be able to design an experiment to evaluate a chemical’s
effectiveness as an antimicrobial. Be able to evaluate the effectiveness if a set of
data from an experiment is provided.
UV: Understand the mechanism of UV damage. How it can be used for bacterial
control. Know how to test the effectiveness of UV against microbes and factors
that may interfere in its antimicrobial activity.
4. Conjugation: Know how bacterial conjugation works and the outcome of the
process. Be able to apply the technique in testing genetic material transfer in
bacteria and analyze results from experiments similar to the one conducted in
class.