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Question 1 1. Many of the larger DNA viruses are able to enhance viral protein translation by coding for ribosomes that selectively translate viral proteins. Answer True False 2 points Question 2 1. A viral messenger RNA has the following sequence: AUG AGU GGG GCC CAU GGG AAA CCC AGG UUU The following proteins are produced: MetAsnGlyAlaHisGlyLysProArgPhe MetGlyAsnProGly What is the mechanism that generates this diversity? Answer A. Polyprotein Synthesis B. Leaky Scanning of Termination D. Ribosomal frameshift C. Suppression 2 points Question 3 1. Some viruses are able to initiate translation at sites other than AUG(Methionine)? Answer True False 2 points Question 4 1. A viral messenger RNA has the following sequence: AUG AGU GGG GCC CAU GGG AAA CCC AGG UUU The following proteins are produced: MetAsnGlyAlaHisGlyLysProArgPhe… MetAsnGlyAlaProTrpGluThrGlnVal… What is the mechanism of diversity in translation of this viral mRNA? Answer A. Ribosomal Frameshift B. Leaky Scanning C. Reinitiation D. Suppression of Termination 2 points Question 5 1. The genomes of (+) RNA viruses can be translated immediately upon cellular entry, even though they do not possess a 5' 7-methylguanosine cap due to the presence of this structure at the 5' end of the genome [x]. Answer 2 points Question 6 1. Most viral infections induce activation of the eIF2a kinase PKR which halts protein translation in an infected cell. Which of the following mechanims enable viruses to evade the antiviral effects of PKR (Select all that apply)? Answer A Viral . B. Viral protein inhibits eIF2a phosphatase. proteins inhibit dsRNA binding to PKR produces its own kinase that phsophorylates eIF2a. Viral protein binds to PKR dsRNA binding site and activates PKR autophosphorylation. C. Virus D . E. Viral proteins act as pseudo-substrates that inhibit PKR kinase activity 2 points Question 7 1. Describe how picornaviruses are able to produce multiple proteins from a single mRNA (Limit 3-5 sentences). Answer Press Tab to enter the content editor. For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). Path: p Words:0 2 points Question 8 1. The figure below depicts an SDS-PAGE protein gel in which total cell protein is visualized at various time points after a viral infection. The protein bands labeled 1A, 2 and VP3 are all viral proteins. Explain in 2-4 sentences what is occurring in this figure and why it is advantageous to the virus Answer Press Tab to enter the content editor. For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). Path: p Words:0 2 points Question 9 1. Match the following with the translation initiation complex Answer A B C Answer Cellular A. Translation Initiation Complex Read Answer Items for B. Question 9 C. Picornavirus Translation Initiation Complex Read Answer Items for Question 9 Flavivirus/Hepatitis C Virus Translation Initiation Complex Read Answer Items for Question 9 2 points Question 10 1. The figure below demonstrates various mechanisms viruses have evolved to inhibit translation of host proteins. Describe in 2-3 sentences each how the picornavirus 3C protein and the HIV protease inhibit cellular host translation. Answer Press Tab to enter the content editor. For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). Path: p Words:0 2 points