General Biology: In molecular biology, which of the following

Subject: Biology / General Biology
Question
In molecular biology, which of the following best reflects what we mean by “cloning DNA”______

a. DNA that has been derived from two or more sources.

b. Causing DNA mutations with X-rays

c. Transcribing the DNA into RNA, to then translate into protein.

d. DNA that has been transferred from an original source into a new one.

You’re trying to clone a 2.3kb insert into a 4kb vector using only one restriction enzyme. Question 1: which of the following band patterns represents your best candidate for successful insertion following digestion of the clones with the same enzyme you used for cloning?

a. a band at 2kb, another one at 3kb and a third at 4kb.

b. a band between 2 and 3kb + a band at 4kb.

c. three bands that correspond to linear, nicked and supercoiled plasmid.

d. a brighter 4kb band (because the vector carries the insert).

You’re trying to clone a 2.3kb insert into a 4kb vector using only one restriction enzyme. Question 2: which of the following band patterns represents your best candidate for successful insertion if you do NOT digest your candidate clones?

a. a band slightly over the 6 kb band marker.

b.a band at 4kb, another slightly over 6kb and a third slightly over 7kb.

c.a band at 2.3kb, and 3 corresponding to linear, nicked and supercoiled vector.

d.none of the other options is correct, it is impossible to infer insertion without digesting your clones.

Which of the following is NOT needed in a PCR reaction?

a.reverse transcriptase.

b.a DNA template.

c.primers flanking the sequence to be amplified.

d.free deoxyribonucleotides (dNTPs).

Which of the following is the most likely disadvantage of using cheaper DNA polymerases in a PCR reaction?

a.you may introduce mutations in the amplified DNA.

b.longer fragments will be more easily amplified.

c.you will obtain more copies of DNA.

d.you will need for longer primers for a successful PCR

Reverse genetics attempts to determine the function of a gene of known sequence but unknown function by any of the following approaches, EXCEPT_____

a. knockdown.

b.site-directed mutagenesis.

c.transgenesis.

d.sequencing.

Restriction endonucleases (aka. restriction enzymes)_____

a. evolved as a defense mechanism in viruses against bacteria.

b.are used to cut RNA at defined sequences.

c.were named for their role inside the nucleus.

d.can be used to create pieces that are cohesive, or sticky.

Cloning a gene after isolating it requires all of the following, EXCEPT________

a. ligation into a vector.

b.transformation of the vectors into recipient cells.

c.identification of the transformants that have the gene of interest.

d.expressing the gene of interest to identify the useful clones through protein detection.

A cloning vectors should include all of the following, EXCEPT______

a.a multiple-cloning site (MCS).

b.a selection marker.

c.a promoter for bacterial expression.

d.one or more origin of replication (ore) sites.

Which of the following should a good eukaryotic expression vector include?

a.a plant cell transformation signal.

b.an operator sequence.

c.a strong TATA box.

d.a transcription terminator sequence]

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