Chromatography Analysis Pigment
Subject: Biology / General Biology
Question
Question 1 (8 points) Complete the following chart. You may have completed "Investigation 5.B" from pages 172 and
173 of the textbook in a laboratory setting. If so, you may complete the following chart using
your own chromatography paper from the investigation. If you were unable to perform the
investigation, use the diagram provided in this assignment to help you fill out the chart. Once
you have filled out the chart, answer questions the next four questions that follow.
Question 1 options:
Chromatography Analysis Pigment
Name
carotenes
chlorophyll A
chlorophyll B
xanthophylls Distance
Distance
Pigment
Travelled by
Travelled by
Colour
Solvent
Pigment (cm)
Front (cm) Reference
Flow (Rf) Question 2 (1 point)
The presence of pigments other than chlorophyll in plants is important because these pigments
Question 2 options:
A) are used to build chlorophyll
B) can use oxygen to carry out photosynthesis
C) function in exactly the same way as chlorophyll
D) allow the plant to utilize wavelengths of light other than those used by chlorophyll
Question 3 (4 points)
Describe two practical uses for an artificial chemical that behaves like chlorophyll. Explain the
value of each of the uses to society.
Question 3 options: Question 4 (1 point)
If a certain band on one chromatography strip has been identified as chlorophyll a, chlorophyll a
could be identified on a second chromatography strip by looking for a band
Question 4 options:
A) with no colour
B) of darker colour
C) in reverse position
D) in a parallel position Question 5 (2 points)
Describe a practical application of chromatography, and give and example that is unrelated to
photosynthesis.
Question 5 options: Question 6 (4 points) Select all possible answers that would complete this statement.
Scientists are investigating ways to produce hydrogen using an artificial photosystem II because
this technology would
Question 6 options:
depend on limited solar energy. combine water (a renewable resource) into hydrogen gas. prevent hydrogen gas, a completely dirty burning fuel, to become available as an alternative
fuel source thus reducing peoples ecological footprint. split water (a renewable resource) into hydrogen gas. be an unstable but abundant resource once it can be efficiently produced. allow hydrogen gas, a completely clean burning fuel, to become available as an alternative
fuel source and allow people to reduce their ecological footprint. depend on unlimited solar energy. be a sustainable resource once it can be efficiently produced.
Question 7 (10 points)
Summarize in point form the events that take place in the light-dependent reactions of
photosynthesis.

