biochemistry

biochemistry

 

biochemistry

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Q Q Q 3 CHE414 Problem Set1 (F17).pdf (page 3 of 3) 7
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3. (3 points each) Congratulations! You have dtscoyered a new protein. You expect the protein to
have a molecular weight of 40 kilodaltons (RD) based on the product of the gene that encodes
it. but when you punfy the protein. it actually has a molecular weight of 200 kD. To add to the lay
confuslon. only the expected polypeptide is present.
a. What the heck is gotng on here? Why might the purtfled protein be larger than the product
encoded by the gene?
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b. Is the primary structure of your 40 kD protein dtfl’erent than the prtmary structure of the
200 kD protein? Explaln your reasoning briefly.
c What about the ternary structure? Do you think the tertiary structure wtll be the same or
different between the 40 RD and 200 kD proteins? Explain your reasoning briefly.
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(1 And let‘s not forget about quaternary structure. Would that be the same or different
between the two protelns? Explain your reasoning briefly.

4. (3 points each) Consider the following changes and describe the effect these changes have on , i» 7
the affinity between oxygen and hemoglobin. Explam your reasoning briefly. ill
a. The pH of blood plasma drops from 7.4 to 7.1 7
b. The BPG concentration tn the blood decreases from 8 mM to 6 mM “m
c A hemoglobin mutauon that substitutes a Glu for an Asp on the surface of the oi subumt
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