1.	You are trying to identify genes that are involved in pyrimidine synthesis in yeast using a forward genetic approach. 
•	How would you go about this?
•	Using the results from the above experiments, how do you characterize these genes molecularly using a reverse genetic approach?
•	In the above experiment you identify several genes that encode for different enzymes involved in a metabolic pathway, which you order using epistasis analysis. However, during these experiments you find that loss-of-function mutations of one particular gene lead to a loss of expression of most of the enzyme-coding genes. What is your interpretation of this result concerning the role and molecular identity of this gene? What is your prediction, and how would you test your hypothesis? 

2.The eukaryotic gene below has multiple promoters (PrI and PrII) and cis-regulatory regions (CRE), in addition to alternatively spliced transcripts (A, B, C) shown below the gene. What consequences can mutations at the indicated locations a-h have? Distinguish between single-nucleotide changes versus structural mutations.