Hi, This for Biomanic. Attached is my assignment….
Hi,
This for Biomanic. Attached is my assignment. Please review and answer.
DNA and Protein synthesis sample.docx Download Attachment
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DNA
and
Synthesis
Protein
Hands-On Labs, Inc. Version 42-005100-01
b e r s i t n
L R s s a t
a
po A
t
This document is not meant to be a substitute for a formal laboratory report. The Lab
Report Assistant is simply a summary of the experiment’s questions, diagrams if
needed, and data tables that should be addressed in a formal lab report. The intent is to
facilitate students’ writing of lab reports by providing this information in an editable file
which can be sent to an instructor.
Exercise 1: Modeling
DNA
The students must attach the DNA model to this lab
report.
Note which nucleotides form pairs—cytosine and guanine, and adenine and thymine
form pairs.
s ns
Qu tio
e
A. The following list indicates the bases on one strand of a DNA molecule. Write in the
names of the bases that could pair with each to make the complementary strand.
cytosine
adenine
cytosine
cytosine
adenine
guanine
thymine
guanine
B. What are the three components of a DNA nucleotide? How do these molecules
connect together?
C. What are the four bases found in DNA? Which of these bases pair together?
Exercise 2: The Globin Gene
r u
P o re
ce d
1. Complete the DNA molecule by writing a complementary strand.
Coding Strand:
CGT CTC TTC GGA CAC
Complementary Strand:
2.
Write the mRNA sequence that will be created in the process of transcription. The
DNA coding strand has the information for the gene, so the strand must be
transcribed. The relationships are slightly different for RNA, because RNA does not
have T; therefore, U should be substituted for T. To transcribe DNA to RNA, the
pairing relationship is A – U, T – A, C – G, and G – C, respectively.
Coding Strand:
CGT CTC TTC GGA CAC
mRNA Strand:
3. codon is recognized, the protein creation is terminated. a reference. Remember, when a “stop”
Translate the mRNA into amino acids. Use Table 1 as
mRNA Strand:
Amino Acids Formed:
Qu sti ns
e o
A. How many nucleotides would it take to construct the mRNA coding strand of the ?subunit of the hemoglobin A molecule?
B. How many nucleotides would it take to model the entire ?-subunit of the hemoglobin A
molecule?
C. Is the ?-subunit of the hemoglobin A molecule a complete DNA molecule
(chromosome) or part of one? Explain.
D. What would happen if one of the DNA nucleotides was deleted? What if the first
T was substituted for an A? Would a substitution always result in a change? Explain why
or why not.
E. Using your newly formed model of DNA from Exercise 1, write the coding strand
below. Use the coding strand to determine the mRNA strand and the amino acids
formed. Do this separately for Row 1 and Row 2 of your DNA model.
Data Table: Newly Formed Model of DNA from Exercise 1
ROW 1
ROW 2
Coding Strand
mRNA Strand
Amino Acids
Formed
F. Did the new DNA model form any two of the same amino acids?
G. Optional: Compare the amino acids that were formed in this experiment with
those of classmates who also performed this experiment. Were there many similarities?